Pharmaceutical Impurity Profile on Luna Omega 1.6 µm C18 50x2.1 mm (Batch 1) using Agilent 1200 UHPLC Binary system

LC Conditions

Column

Luna Omega 1.6µm C18 100Å, LC Column 50 x 2.1 mm, EA

Brand

Luna Omega

Part No

00B-4742-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in Water

B: 0.1% Formic Acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

98

2

2

0.5

98

2

3

10.5

65

35

4

11

65

35

5

11.5

98

2

6

15

98

2

Flow Rate

0.40 mL/min

Temperature

30°C

System

Agilent Technologies Agilent Technologies 1200 Series Binary LC System

Detection

LC/UV (DAD, PDA) @ 254 nm (nanometers)

Detection Info