Pharmaceutical Impurity Profile on Luna Omega 3 µm C18 (batch 3) 150x4.6 mm using Agilent 1200 UHPLC Binary system

LC Conditions

Column

Luna Omega 3 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna Omega

Part No

00F-4784-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in Water

B: 0.1% Formic Acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

98

2

2

1.5

98

2

3

31.5

65

35

4

33

65

35

5

34.5

98

2

6

37

98

2

Flow Rate

2.00 mL/min

Temperature

30°C

System

Agilent Technologies Agilent Technologies 1200 Series Binary LC System

Detection

LC/UV (DAD, PDA) @ 254 nm (nanometers)

Detection Info