Micro-LC Phase Selectivity of Peptides

LC Conditions

Column

Kinetex 2.6u F5 100A, LC Column 150 x 0.3 mm, Ea

Brand

Kinetex

Part No

00F-4723-AC

Phase Name

F5

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in Water

B: 0.1% Formic Acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

98

2

2

60

60

40

3

60.1

20

80

4

65

20

80

5

65.1

98

2

6

70

98

2

Flow Rate

10.00 mL/min

Temperature

40°C

System

Sciex Thermo Fisher LC/MS System

Detection

LC/MS @ (250°C)

Detection Info