EuPH Teriflunomide Related Substances Reference Solution C on Kinetex 2.6u C18 150x4.6mm using a Waters H-Class Bio UHPLC system

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4462-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Acetonitrile and Buffer (10:90, v/v)

B: Buffer and Acetonitrile (10:90, v/v)

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

76

24

2

2.55

76

24

3

12.18

23

77

4

13.14

76

24

5

17

76

24

Flow Rate

1.04 mL/min

Temperature

40°C

System

Waters ACQUITY UPLC H-Class Waters ACQUITY UPLC H-Class

Detection

LC/UV (DAD, PDA) @ 249 nm (nanometers)

Detection Info