PhEur Atazanavir Sulfate - Assay; Reference solution C on Luna 3u C18(2) 150x4.6mm using Agilent 1260 Quaternary UHPLC system

LC Conditions

Column

Luna 3 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4251-E0

Phase Name

C18(2)

Elution Type

Isocratic

Mobile Phase

A: mix 25 volumes of acetonitrile and 75 volumes of a freshly prepared 2.73 g/L solution of potassium dihydrogen phosphate previously adjusted to pH 3.5 with dilute phosphoric acid.

B: mix 25 volumes of a freshly prepared 2.73 g/L solution of potassium dihydrogen phosphate previously adjusted to pH 3.5 with dilute phosphoric acid, and 75 volumes of acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

50

50

Flow Rate

1.00 mL/min

Temperature

25°C

System

Agilent Technologies 1260 Infinity Agilent Technologies 1200 Series Quaternary LC System

Detection

LC/UV (DAD, PDA) @ 215 nm (nanometers)

Detection Info