PhEur Atazanavir Sulfate - Impurity K; Reference solution D on Luna Omega 3u C18 150x4.6mm using Agilent 1260 Quaternary UHPLC system

LC Conditions

Column

Luna Omega 3 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna Omega

Part No

00F-4784-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: mix 25 volumes of acetonitrile and 75 volumes of a freshly prepared 2.73 g/L solution of potassium dihydrogen phosphate previously adjusted to pH 3.5 with dilute phosphoric acid.

B: mix 25 volumes of a freshly prepared 2.73 g/L solution of potassium dihydrogen phosphate previously adjusted to pH 3.5 with dilute phosphoric acid, and 75 volumes of acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

5.6

95

5

3

8.6

0

100

4

14.6

0

100

5

16

95

5

6

23

95

5

Flow Rate

1.00 mL/min

Temperature

25°C

System

Agilent Technologies 1260 Infinity Agilent Technologies 1200 Series Quaternary LC System

Detection

LC/UV (DAD, PDA) @ 215 nm (nanometers)

Detection Info