USP Tigecycline Assay Standard Solution on Luna 5u C18(2) 150x4.6mm using Agilent 1260 Quaternary UHPLC system

LC Conditions

Column

Luna 5 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4252-E0

Phase Name

C18(2)

Elution Type

Isocratic

Mobile Phase

A: Acetonitrile:Buffer (140:860) Buffer: 4.35g/L of dibasic Potassium Phosphate and 0.93 g/L of EDTA, adjusted with Phosphoric acid to a pH of 6.2

Pre-mixed

Gradient Profile

Step No.

Time(min)

%A

%B

1

45

100

Flow Rate

1.00 mL/min

Temperature

30°C

System

Agilent Technologies 1260 Infinity Agilent Technologies 1200 Series Quaternary LC System

Detection

UV-Vis Abs.-Diode Array (PDA) @ 248 nm (nanometers)

Detection Info