Fexofenadine Hydrochloride Reference Solution (c) for Related substances and assay as per Ph. Eur. Monograph 2280

LC Conditions

Column

Kinetex 5 µm Phenyl-Hexyl 100 Å, LC Column 250 x 4.6 mm, Ea

Brand

Kinetex

Part No

00G-4603-E0

Phase Name

Phenyl-Hexyl

Elution Type

Isocratic

Mobile Phase

A: Acetonitrile: Buffer: TEA (350:650:3) v/v/v ( Buffer: 6.64g/L of Monobasic Sodium Phosphate and 0.84g/L of Sodium perchlorate in water pH:2.00 with orthophosphoric acid.)

Gradient Profile

Step No.

Time(min)

%A

%B

1

50

100

Flow Rate

1.50 mL/min

Temperature

25°C

System

Waters Waters HPLC System

Detection

LC/UV (DAD, PDA) @ 220

Detection Info