AAV9 FLD at 0.5 mL/min Flow Rate on a Biozen dSEC-7

LC Conditions

Column

Biozen 3 µm dSEC-7, LC Column 150 x 4.6 mm, Ea

Brand

Biozen LC

Part No

00F-4789-E0

Phase Name

dSEC-7

Elution Type

Gradient

Mobile Phase

A: 250 mM NaCl + 5 mM KCl + 20 mM Sodium phosphate 0.001% Pluronics F68

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

2

20

100

Flow Rate

0.50 mL/min

Temperature

30°C

System

Agilent Technologies 1260 Infinity Agilent Technologies 1260 Infinity

Detection

LC/FLD (Fluorescence Detector) @ 280 nm (nanometers)

Detection Info

Sample Notes

1xPBS with 0.001% Pluronics F68