Tacrolimus Standard solution five replicated injections 3mg/ml. USP-Assay

LC Conditions

Column

Luna Omega 3 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna Omega

Part No

00F-4784-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Solution-A: 6mM Phosphoric acid in milliQ water. (0.4ml of 85% Orthophosphoric acid in 1000ml of water, filtered through 0.22µm membrane nylon filter)

Solution-B: A Mixture of Acetonitrile tert-butyl methyl in ratio of 810:190ml v/v. Solution A and Solution B in a ratio of 4:1 (400:100 ml v/v)

B: Solution-A: 6mM Phosphoric acid in milliQ water. (0.4ml of 85% Orthophosphoric acid in 1000ml of water, filtered through 0.22µm membrane nylon filter)

Solution-B: A Mixture of Acetonitrile tert-butyl methyl in ratio of 810:190ml v/v. Solution A and Solution B in a ratio of 1:4 (100:400 ml v/v)

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

72

28

2

30

72

28

3

53

15

85

4

54

72

28

5

60

72

28

Flow Rate

1.50 mL/min

Temperature

60°C

System

Waters Arc HPLC Waters HPLC System

Detection

LC/UV (DAD, PDA) @ 220 nm (nanometers)

Detection Info