USP Abacavir and Lamivudine Tablets - Assay (System Suitability Solution)

LC Conditions

Column

Luna Omega 3 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna Omega

Part No

00F-4784-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Water and TFA (2000:1)

B: Acetonitrile, Methanol and TFA (1000:1000:1)

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

2

3.43

100

0

3

10.29

70

30

4

10.37

40

60

5

11.23

40

60

6

11.31

100

0

7

18.31

100

0

Flow Rate

1.50 mL/min

Temperature

40°C

System

Agilent Technologies 1200 Series Agilent Technologies 1200 Series

Detection

LC/UV (DAD, PDA) @ 270 nm (nanometers)

Detection Info

Sample Notes

USP Lamivudine Resolution Mixture C RS (0.8mg) in 2.5mL 0.1N hydrochloric acid