USP Abacavir and Lamivudine Tablets - Assay (System Suitability Solution)

LC Conditions

Column

Kinetex 2.6 µm EVO C18 100 Å, LC Column 100 x 4.6 mm, EA

Brand

Kinetex

Part No

00D-4725-E0

Phase Name

EVO C18

Elution Type

Gradient

Mobile Phase

A: Water and TFA (2000:1)

B: Acetonitrile, Methanol and TFA (1000:1000:1)

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

2

1.98

100

0

3

5.94

70

30

4

5.99

40

60

5

6.49

40

60

6

6.54

100

0

7

13

100

0

Flow Rate

1.50 mL/min

Temperature

40°C

System

Agilent Technologies 1200 Series Agilent Technologies 1200 Series

Detection

LC/UV (DAD, PDA) @ 270 nm (nanometers)

Detection Info

Sample Notes

USP Lamivudine Resolution Mixture C RS (0.8mg) in 2.5mL 0.1N hydrochloric acid