USP Atazanavir Sulfate (Assay, Organic Impurities – Procedures 1 and 2, Limit of Atazanavir Related Compound A)​. Atazanavir system suitability solution for USP-Assay

LC Conditions

Column

Luna Omega 3 µm Polar C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna Omega

Part No

00F-4760-E0

Phase Name

Polar C18

Elution Type

Isocratic

Mobile Phase

A: Buffer: 2.73 g/L of monobasic potassium phosphate in water. Adjusted pH 3.50 with Orthophosphoric acid (Diluted 1ml to 10 water).

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

15

50

50

Flow Rate

1.00 mL/min

Temperature

25°C

System

Waters ACQUITY UPLC H-Class Waters ACQUITY UPLC H-Class

Detection

UV-Vis Abs.-Diode Array (PDA) @ 215

Detection Info