USP Amlodipine, Valsartan, and Hydrochlorothiazide Tablets –Assay and Impurities- Amlodipine,Valsartan,Hydrochlorothiazide System suitability solution Chromatogram USP-Impurities

LC Conditions

Column

Luna Omega 3 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna Omega

Part No

00F-4784-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Orthophosphoric acid in water. (1 ml of Orthophosphoric acid in dissolved in 1000 ml of water. Filtered through 0.22µ Nylon filter. Mobile phase A: Acetonitrile: water and Phosphoric acid in ratio of 50:950:1 ml v/v/v.

B: 0.1% Orthophosphoric acid in water. (1 ml of Orthophosphoric acid in dissolved in 1000 ml of water. Filtered through 0.22µ Nylon filter. Mobile phase B: Acetonitrile: water and Phosphoric acid in ratio of 950:50:1 ml v/v/v.

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

3

50

50

3

6

40

60

4

10

5

95

5

10.1

95

5

6

15

95

5

Flow Rate

1.50 mL/min

Temperature

40°C

System

Waters ACQUITY UPLC H-Class Waters ACQUITY UPLC H-Class

Detection

LC/UV (DAD, PDA) @ 210

Detection Info