USP Amlodipine and Olmesartan Medoxomil Tablets - Standard solution - Kinetex Biphenyl

LC Conditions

Column

Kinetex 5 µm Biphenyl 100 Å, LC Column 250 x 4.6 mm, Ea

Brand

Kinetex

Part No

00G-4627-E0

Phase Name

Biphenyl

Elution Type

Gradient

Mobile Phase

A: 6.9 g/L of Sodium phosphate buffer, monobasic, pH 2.5

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

68

32

2

12

68

32

3

15

30

70

4

21

30

70

5

23

68

32

6

25

68

32

Flow Rate

2.00 mL/min

Temperature

60°C

System

Agilent Technologies Agilent Technologies 1200 Series Binary LC System

Detection

LC/UV (DAD, PDA) @ 254 nm (nanometers)

Detection Info