HILIC Separation of Catecholamines
LC Conditions
Column
Luna Omega 3µm HILIC 100Å, LC Column 150 x 4.6 mm, ea
Brand
Luna Omega
Part No
00F-4808-E0
Phase Name
HILIC
Elution Type
Isocratic
Mobile Phase
A: Water
B: 100 mM Ammonium Formate, pH 3.2
C: Acetonitrile
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
5
10
Flow Rate
1.00 mL/min
Temperature
23°C
System
Waters ACQUITY UPLC H-Class Waters ACQUITY UPLC H-Class
Detection
LC/UV (DAD, PDA) @ 270 nm (nanometers)
Detection Info
Sample Notes
Sample solvent: Acetonitrile: Water 90:10 Toluene 0.54 µL/mL 3-Methoxytyramine 60 µg/mL Serotonin 30 µg/mL Dopamine 80 µg/mL
Order Items Used in This Application
- 1. Toluene
- 2. 3-Methoxytyramine
- 3. Serotonin
- 4. Dopamine
-OEChem-06211003142D<br><br> 15 15 0 0 0 0 0 0 0999 V2000<br> 2.8660 0.5000 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 0.0000 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 0.0000 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 1.5000 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -1.0000 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 -1.0000 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 -1.5000 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.4631 0.3100 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2690 0.3100 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4860 1.5000 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 2.1200 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.2460 1.5000 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.4631 -1.3100 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2690 -1.3100 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 -2.1200 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 2 2 0 0 0 0<br> 1 3 1 0 0 0 0<br> 1 4 1 0 0 0 0<br> 2 5 1 0 0 0 0<br> 2 8 1 0 0 0 0<br> 3 6 2 0 0 0 0<br> 3 9 1 0 0 0 0<br> 4 10 1 0 0 0 0<br> 4 11 1 0 0 0 0<br> 4 12 1 0 0 0 0<br> 5 7 2 0 0 0 0<br> 5 13 1 0 0 0 0<br> 6 7 1 0 0 0 0<br> 6 14 1 0 0 0 0<br> 7 15 1 0 0 0 0<br>M END
Compound Name
Toluene
CID
1140
Molecular Formula
C7H8
Molecular Weight
92
Similar Applications
Hydrophilic Interaction
HILIC Separation of water-soluble vitamins
Luna Omega 3µm HILIC 100Å, LC Column 150 x 4.6 mm, ea
28699
Hydrophilic Interaction
HILIC Separation of Catecholamines
Luna Omega 3µm HILIC 100Å, LC Column 150 x 4.6 mm, ea
28692