HILIC Separation of water-soluble vitamins

LC Conditions
Column

Luna Omega 3µm HILIC 100Å, LC Column 150 x 4.6 mm, ea

Brand

Luna Omega

Part No

00F-4808-E0

Phase Name

HILIC

Elution Type

Isocratic

Mobile Phase

A: 100 mM Ammonium Formate, pH 3.2

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

10

90

Flow Rate

1.00 mL/min

Temperature

23°C

System

Waters ACQUITY UPLC H-Class Waters ACQUITY UPLC H-Class

Detection

LC/UV (DAD, PDA) @ 260 nm (nanometers)

Detection Info

Order Items Used in This Application

Luna Omega 3µm HILIC 100Å, LC Column 150 x 4.6 mm, ea
00F-4808-E0

Luna Omega 3µm HILIC 100Å, LC Column 150 x 4.6 mm, ea

View Product

  • 1. Toluene
  • 2. Nicotinamide
  • 3. Pyridoxine
  • 4. PABA
  • 5. Nicotinic acid
  • 6. Thiamine
-OEChem-06211003142D<br><br> 15 15  0     0  0  0  0  0  0999 V2000<br>    2.8660    0.5000    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.0000    0.0000    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.7320    0.0000    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.8660    1.5000    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.0000   -1.0000    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.7320   -1.0000    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.8660   -1.5000    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    1.4631    0.3100    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.2690    0.3100    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.4860    1.5000    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.8660    2.1200    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.2460    1.5000    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    1.4631   -1.3100    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.2690   -1.3100    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.8660   -2.1200    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>  1  2  2  0  0  0  0<br>  1  3  1  0  0  0  0<br>  1  4  1  0  0  0  0<br>  2  5  1  0  0  0  0<br>  2  8  1  0  0  0  0<br>  3  6  2  0  0  0  0<br>  3  9  1  0  0  0  0<br>  4 10  1  0  0  0  0<br>  4 11  1  0  0  0  0<br>  4 12  1  0  0  0  0<br>  5  7  2  0  0  0  0<br>  5 13  1  0  0  0  0<br>  6  7  1  0  0  0  0<br>  6 14  1  0  0  0  0<br>  7 15  1  0  0  0  0<br>M  END
Compound Name

Toluene

CID

1140

Molecular Formula

C7H8

Molecular Weight

92

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