HILIC Separation of water-soluble vitamins

LC Conditions

Column

Luna Omega 3µm HILIC 100Å, LC Column 150 x 4.6 mm, ea

Brand

Luna Omega

Part No

00F-4808-E0

Phase Name

HILIC

Elution Type

Isocratic

Mobile Phase

A: 100 mM Ammonium Formate, pH 3.2

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

10

90

Flow Rate

1.00 mL/min

Temperature

23°C

System

Waters ACQUITY UPLC H-Class Waters ACQUITY UPLC H-Class

Detection

LC/UV (DAD, PDA) @ 260 nm (nanometers)

Detection Info