HILIC Separation of Glucosinates

LC Conditions

Column

Luna Omega 3µm HILIC 100Å, LC Column 150 x 4.6 mm, ea

Brand

Luna Omega

Part No

00F-4808-E0

Phase Name

HILIC

Elution Type

Isocratic

Mobile Phase

A: 30 mM Ammonium Formate in Acetonitrile: Water 85:15, pH ~7 pH 8.88

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

10

90

Flow Rate

2.00 mL/min

Temperature

40°C

System

Waters ACQUITY UPLC H-Class Waters ACQUITY UPLC H-Class

Detection

LC/UV (DAD, PDA) @ 230 nm (nanometers)

Detection Info