Fluoxetine HCl - USP Method

LC Conditions

Official Method

USP L07

Column

Luna 5 µm C8(2) 100 Å, LC Column 250 x 4.6 mm, Ea

Brand

Luna

Part No

00G-4249-E0

Phase Name

C8(2)

Elution Type

Isocratic

Mobile Phase

A: 1%TEA buffer pH 6.0 w/phosphoric acid, pH 6.0

B: Tetrahydrofuran

C: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

60

30

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C8 4 x 3.0mm, 10/Pk Part No.: AJ0-4290 - Holder Part No.: KJ0-4282