RNA Fragments

LC Conditions

Column

Jupiter 5 µm C4 300 Å, LC Column 250 x 4.6 mm, Ea

Brand

Jupiter

Part No

00G-4167-E0

Phase Name

C4

Elution Type

Gradient

Mobile Phase

A: 0.1 M K3PO4 in 0.75% isopropanol, pH 6.8

B: 1.0 M (NH4)SO4 + 0.1M K3PO4 in 0.75% isopropanol, pH 6.8

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

0

100

2

20

70

30

3

30

100

0

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Widepore C4 4 x 3.0mm, 10/Pk Part No.: AJ0-4330 - Holder Part No.: KJ0-4282