Proteins II

LC Conditions

Column

Jupiter 5 µm C4 300 Å, LC Column 50 x 4.6 mm, Ea

Brand

Jupiter

Part No

00B-4167-E0

Phase Name

C4

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA in water

B: 0.1% TFA in acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

2

1

80

20

3

2.5

65

35

4

4

53.5

46.5

5

6

53.5

46.5

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Widepore C4 4 x 3.0mm, 10/Pk Part No.: AJ0-4330 - Holder Part No.: KJ0-4282