Water-Soluble Vitamins - Sphereclone vs. Spherisorb Comparison

LC Conditions

Column

SphereClone 5 µm ODS(2) 80 Å, LC Column 150 x 4.6 mm, Ea

Brand

SphereClone

Part No

00F-4144-E0

Phase Name

ODS(2)

Elution Type

Gradient

Mobile Phase

A: 5 mM Sodium 1-Pentanesulfonate in 0.1% H3PO4

B: 5 mM Sodium 1-Pentanesulfonate in 0.1% H3PO4 in 80% acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97.5

2.5

2

20

70

30

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282