Dabsyl Derivated Phospho Amino Acids
LC Conditions
Column
Ultracarb 5 µm ODS (30) 60 Å, LC Column 250 x 4.6 mm, Ea
Brand
Ultracarb
Part No
00G-0351-E0
Phase Name
ODS (30)
Elution Type
Gradient
Mobile Phase
A: 20 mM Sodium bicarbonate pH 8.1 with 4% dimethylformamide
B: (90:10) Acetonitrile/2-Propanol
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
82
18
2
6
75
25
3
26
60
40
4
41
30
70
5
43
30
70
Flow Rate
1.00 mL/min
Temperature
22°C
Detection
UV-Vis Abs.-Variable Wave.(UV) @ (22°C)
Detection Info
Sample Notes
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282
Order Items Used in This Application
- 1. Phosphothreonine
- 2. Phosphoserine
- 3. Phosphotyrosine
- 4. Aspartic acid
- 5. Glutamic acid
- 6. Carboxymethylcysteine
- 7. Serine
- 8. Threonine
- 9. Glycine
- 10. Alanine
- 11. Proline
- 12. Valine
- 13. Methionine
- 14. Isoleucine
- 15. Leucine
- 16. Arginine
- 17. Phenylalanine
- 18. Cysteine
- 19. Ammonia
- 20. Lysine
- 21. Histidine
- 22. Dityrosine
- 23. Tyrosine
Similar Applications
No Similar applications found.