Hydrocortisone Acetate - USP Method

LC Conditions

Official Method

USP L03

Column

Luna 10 µm Silica (2) 100 Å, LC Column 250 x 4.6 mm, Ea

Brand

Luna

Part No

00G-4091-E0

Phase Name

Silica(2)

Elution Type

Isocratic

Mobile Phase

A: Butyl chloride:water-saturated butyl chloridemethanol:glacial acetic acid (95:95:14:7:6)

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Silica 4 x 3.0mm, 10/Pk Part No.: AJ0-4348 - Holder Part No.: KJ0-4282