Hydrocodone Bitartrate - USP Acceptable Modified Method

LC Conditions

Column

Luna 5 µm Silica (2) 100 Å, LC Column 250 x 4.6 mm, Ea

Brand

Luna

Part No

00G-4274-E0

Phase Name

Silica(2)

Elution Type

Isocratic

Mobile Phase

A: acetonitrilediethylamine:methanol (440/2.2/0.55/45 v/v/v/v)

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

Flow Rate

1.50 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Silica 4 x 3.0mm, 10/Pk Part No.: AJ0-4348 - Holder Part No.: KJ0-4282