Red Clover Isoflavones - HPLC

LC Conditions

Column

Prodigy 5 µm ODS-3 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Prodigy

Part No

00F-4097-E0

Phase Name

ODS-3

Elution Type

Gradient

Mobile Phase

A: Water + 0.1% phosphoric acid

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

22

42

58

Flow Rate

1.00 mL/min

Temperature

45°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282