Amino Acids

LC Conditions

Column

Luna 3 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4251-E0

Phase Name

C18(2)

Elution Type

Gradient

Mobile Phase

A: 4 mM CH3COO-Na+, 4% N,N-Dimethylformamide, pH 6.5 adjusted with acetic acid

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

80

20

2

20

60

40

3

28

30

70

4

38

30

70

5

40

80

20

Flow Rate

0.75 mL/min

Temperature

40°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282