St. John's Wort on Synergi Max-RP
LC Conditions
Column
Synergi 4 µm Max-RP 80 Å, LC Column 150 x 4.6 mm, Ea
Brand
Synergi
Part No
00F-4337-E0
Phase Name
Max-RP
Elution Type
Isocratic
Mobile Phase
A: 100mM Ammonium acetate
B: Acetonitrile
C: Methanol
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
20
50
Flow Rate
1.50 mL/min
Temperature
22°C
Detection
UV-Vis Abs.-Variable Wave.(UV) @ (22°C)
Detection Info
Sample Notes
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074 - Holder Part No.: KJ0-4282
Order Items Used in This Application
- 1. Pseudohypericin
- 2. Hypericin
-OEChem-05081000432D<br><br> 55 62 0 0 0 0 0 0 0999 V2000<br> 9.5494 -1.3020 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4890 2.1969 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.7654 -2.8388 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6657 3.1814 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.8136 3.6733 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5501 0.7496 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.4039 0.1582 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3263 3.6671 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.2566 -3.9679 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.9348 0.1672 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0688 0.6672 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.9508 -0.8743 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1588 0.1604 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.8008 0.6672 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0688 1.6672 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0528 -1.4020 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1507 -0.8812 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.8008 1.6672 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.9348 2.1672 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.8280 -1.3370 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3194 0.6886 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6603 0.1725 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.2105 2.1642 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0789 -2.4435 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2357 -1.3794 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6737 -0.8192 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3450 1.6800 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.8781 -2.3776 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6716 2.1814 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.9447 3.1784 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3932 0.2118 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.5427 0.6664 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1970 3.1753 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.9977 -2.9346 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3510 -0.8292 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.5484 1.6776 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0700 3.6859 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.2277 -2.9683 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2068 -2.3790 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.0204 -3.5542 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8031 -1.1194 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.0859 1.9866 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0688 4.3059 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.9989 -2.3921 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.6204 -3.0936 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.5871 -2.3610 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.1889 -2.9987 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.8266 -2.3969 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.7929 -3.4582 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.2008 3.4946 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.8173 4.2933 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 0.4637 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.9438 0.4630 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3204 4.2871 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7288 -4.2933 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 26 2 0 0 0 0<br> 2 27 2 0 0 0 0<br> 3 28 1 0 0 0 0<br> 3 49 1 0 0 0 0<br> 4 29 1 0 0 0 0<br> 4 50 1 0 0 0 0<br> 5 30 1 0 0 0 0<br> 5 51 1 0 0 0 0<br> 6 31 1 0 0 0 0<br> 6 52 1 0 0 0 0<br> 7 32 1 0 0 0 0<br> 7 53 1 0 0 0 0<br> 8 33 1 0 0 0 0<br> 8 54 1 0 0 0 0<br> 9 38 1 0 0 0 0<br> 9 55 1 0 0 0 0<br> 10 11 1 0 0 0 0<br> 10 12 2 0 0 0 0<br> 10 14 1 0 0 0 0<br> 11 13 2 0 0 0 0<br> 11 15 1 0 0 0 0<br> 12 16 1 0 0 0 0<br> 12 20 1 0 0 0 0<br> 13 17 1 0 0 0 0<br> 13 21 1 0 0 0 0<br> 14 18 2 0 0 0 0<br> 14 22 1 0 0 0 0<br> 15 19 2 0 0 0 0<br> 15 23 1 0 0 0 0<br> 16 17 2 0 0 0 0<br> 16 24 1 0 0 0 0<br> 17 25 1 0 0 0 0<br> 18 19 1 0 0 0 0<br> 18 29 1 0 0 0 0<br> 19 30 1 0 0 0 0<br> 20 26 1 0 0 0 0<br> 20 28 2 0 0 0 0<br> 21 27 1 0 0 0 0<br> 21 31 2 0 0 0 0<br> 22 26 1 0 0 0 0<br> 22 32 2 0 0 0 0<br> 23 27 1 0 0 0 0<br> 23 33 2 0 0 0 0<br> 24 34 2 0 0 0 0<br> 24 38 1 0 0 0 0<br> 25 35 2 0 0 0 0<br> 25 39 1 0 0 0 0<br> 28 34 1 0 0 0 0<br> 29 36 2 0 0 0 0<br> 30 37 2 0 0 0 0<br> 31 35 1 0 0 0 0<br> 32 36 1 0 0 0 0<br> 33 37 1 0 0 0 0<br> 34 40 1 0 0 0 0<br> 35 41 1 0 0 0 0<br> 36 42 1 0 0 0 0<br> 37 43 1 0 0 0 0<br> 38 44 1 0 0 0 0<br> 38 45 1 0 0 0 0<br> 39 46 1 0 0 0 0<br> 39 47 1 0 0 0 0<br> 39 48 1 0 0 0 0<br>M END
Compound Name
Pseudohypericin
CID
5281751
Molecular Formula
C30H16O9
Molecular Weight
520
No. Hydrogen Bond Acceptors
9
No. Hydrogen Bond Donors
7
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