Protopin in Fumitory (Earthsmoke)

LC Conditions

Column

Luna 3 µm C18(2) 100 Å, LC Column 75 x 4.6 mm, Ea

Brand

Luna

Part No

00C-4251-E0

Phase Name

C18(2)

Elution Type

Gradient

Mobile Phase

A: 0.005M octanesulfonic acid in water

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

79

21

2

14

76

24

3

20

71

29

4

30

63

37

5

40

51

49

6

45

51

49

7

47

79

21

8

65

79

21

Flow Rate

0.80 mL/min

Temperature

25°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (25°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282