Extraction of Antidepressants on Strata™ X

LC Conditions

Column

Luna 5 µm C8(2) 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4249-E0

Phase Name

C8(2)

Elution Type

Isocratic

Mobile Phase

A: 20 mM KH2PO4, pH 7.0

B: Acetonitrile

C: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

40

30

Flow Rate

2.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Fixed Wave. (UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C8 4 x 3.0mm, 10/Pk Part No.: AJ0-4290 - Holder Part No.: KJ0-4282

Sample Preparation Details

Description

Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk

Part No

8B-S100-TAK

Pretreatment Note

Blow down to almost dryness under slow stream of N2. Reconstitute with mobile phase to 200 µL. Inject 50 uL on HPLC

Condition 1

1.0 mL Methanol

Condition 2

1.0 mL Water

Load

Load 1.00 mL Sample diluted in Buffer

Wash 1

1.0 mL 5% methanol/D. I. water

Dry

1min at 10'' of Hg

Elution 1

1.0 mL Methanol

Evaporation

Evaporate to dryness

Reconstitute

2 mL Mobile Phase