Tryptic Digests of three genetic variants of cytochrome c proteins on Jupiter Proteo LC column

LC Conditions

Column

Jupiter 4 µm Proteo 90 Å, LC Column 250 x 4.6 mm, Ea

Brand

Jupiter

Part No

00G-4396-E0

Phase Name

Proteo

Elution Type

Gradient

Mobile Phase

A: 0.12% TFA in water

B: 0.10% TFA in MeCN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

50

45

55

3

55

5

95

4

60

95

5

5

65

95

5

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (25°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074 - Holder Part No.: KJ0-4282