Anion-exchange of siRNA on Luna NH2

LC Conditions

Column

Luna 5 µm NH2 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4378-E0

Phase Name

NH2

Elution Type

Gradient

Mobile Phase

A: 20mM Tris-HCl

B: 20mM Tris-HCl + 1M NaCl

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

25

0

100

Flow Rate

1.00 mL/min

Temperature

40°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, NH2 4 x 3.0mm, 10/Pk Part No.: AJ0-4302 - Holder Part No.: KJ0-4282