Benzodiazepines (SPE-Plasma) on Hybrid C18 Column

LC Conditions

Column

Gemini 5 µm C18 110 Å, LC Column 150 x 3 mm, Ea

Brand

Gemini

Part No

00F-4435-Y0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic acid in Water

B: 0.1% Formic acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

80

20

2

8

20

80

Flow Rate

0.80 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini C18 4 x 2.0mm, 10/Pk Part No.: AJ0-7596 - Holder Part No.: KJ0-4282

Sample Preparation Details

Description

Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk

Part No

8B-S100-TAK

Condition 1

1mL Methanol

Condition 2

1mL Water

Sample Pretreatment

SPE: Strata X 30mg/ml Sample: 0.5ml human plasam + 0.5ml water + 200ng benzodiazepines Condition: 1ml MeOH, 1ml Water Washing: 1ml water, 1ml 40%MeOH in water Elution: 1ml MeOH Reconstitution: 100ul water

Load

Load 1.00 mL Sample diluted in Water

Wash 1

1mL Water

Wash 2

1mL 40% Methanol in Water

Elution 1

1mL Methanol

Evaporation

Evaporate to dryness

Reconstitute

100 µL Water