1µmole DNA Cartridge Purification - Crude & Elution Fractions

LC Conditions

Column

Clarity 3 µm Oligo-RP, LC Column 50 x 4.6 mm, Ea

Brand

Clarity LC

Part No

00B-4441-E0

Phase Name

Oligo-RP

Elution Type

Gradient

Mobile Phase

A: 50mM TEAA/5% Acetonitrile

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

20

40

60

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Clarity Oligo-RP 4 x 3.0mm, 10/Pk Part No.: AJ0-8135 - Holder Part No.: KJ0-4282

Sample Preparation Details

Condition 1

1.5 mL 50% Methanol/ Water at 1 mL/min

Condition 2

2 mL Clarity Triophilic 3:1 Buffer, 3 times of at 1 mL/min

Load

Load 1.00 mL Sample diluted in Clarity Triophilic 3:1 Buffer

Wash 1

6 mL Clarity Triophilic 3:1 Buffer, 3 times of at 1 mL/min

Wash 2

1.5 mL 2% TFA/50mM NaCl, 2 times of at 1 mL/min

Elution 1

1 mL Methanol / Nuclease-free Water / Acetonitrile (40/40/20) at 1 mL/min

Evaporation

Evaporate to dryness