Applications
Dye-labeled DNA Desalting - Crude, Load, Wash, Elution Fractions
15991
Separation Mode: Reversed Phase
Reversed Phase
Oligo-RP
HPLC
Life Science
Pharmaceutical/Biopharmaceutical
Oligonucleotide
Biochemical Compound / Nutrient

Applications

Dye-labeled DNA Desalting - Crude, Load, Wash, Elution Fractions
Analytes
1

Analyte not found

Details
LC Conditions (App ID: 15991)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 50mM TEAA/5% Acetonitrile
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
109010
2204060
Flow Rate:
1 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 260 nm (22°C)
Sample Preparation Details
Description:
Clarity RP Desalting, 200 mg / 3 mL, Tubes, 50/Pk
Part Number:
Condition 1:
3 mL Methanol at 1 mL/min
Condition 2:
3 mL Water at 1 mL/min
Load:
Load 2.00 mL Sample diluted in 0.2M TEAA
Wash 1:
3 mL 50mM TEAA/5% Acetonitrile at 1 mL/min
Elution 1:
1 mL Methanol/Water/Acetonitrile (40/40/20) at 1 mL/min
Evaporation:
Evaporate to dryness

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