Overlay of PEGylated vs. Native Proteins on Jupiter® 300 C18 Column

LC Conditions

Column

Jupiter 5 µm C18 300 Å, LC Column 150 x 4.6 mm, Ea

Brand

Jupiter

Part No

00F-4053-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA and 2% ACN in Water

B: 70/20% ACN/IPA, 0.08% TFA in Water

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

85

15

2

25

30

70

Flow Rate

1.00 mL/min

Temperature

45°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

Reversed phase of PEGylated and native proteins run on a Jupiter 300 C4 column. Note the decrease in resolution of PEGylated form versus the C4 column.