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Applications
JupiterĀ® 300 C4 - Effect of Varying Mobile Phase Gradient Conditions
16197
Separation Mode: Reversed Phase
Reversed Phase
C4
HPLC
Pharmaceutical/Biopharmaceutical
Life Science
Biochemical - Polymeric
Biochemical Compound / Nutrient

JupiterĀ® 300 C4 - Effect of Varying Mobile Phase Gradient Conditions

Analytes
1PEGylated Ribonuclease A
Details
LC Conditions (App ID: 16197)
Column:
Brand Name:
Jupiter
Part No:
Phase Name:
C4
Sample Note:
Reversed phase runs of PEGylated ribonuclease A using different gradient conditions on a Jupiter 300 C4 column (gradient length 25 min). Note that if initial %B is increased much above 25% then retention and resolution of PEGylated forms is lost. Gradient slopes of between 1-2%/min appear to be a good compromise between resolution and run time.
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