Jupiter® 300 C4 - PEGylation Reaction Time Course (Ribonuclease A)

LC Conditions

Column

Jupiter 5 µm C4 300 Å, LC Column 150 x 4.6 mm, Ea

Brand

Jupiter

Part No

00F-4167-E0

Phase Name

C4

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA in Water

B: 90% ACN 0.09% TFA in Water

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

80

20

2

25

45

55

Flow Rate

1.00 mL/min

Temperature

45°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

PEGylation time course study of ribonuclease. Different time points from a PEGylation reaction of ribonuclease A were taken: unreacted, 0 min., 3 min., 9 min., 30 min., 90 min., and 120 min. Note the increase of greater retaining PEGylation forms over time.