Comparison of Water-Soluble Vitamins: Luna 5µm HILIC 80/20 ACN/Buffer, pH 3.2

LC Conditions

Column

Luna 5 µm HILIC 200 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4450-E0

Phase Name

HILIC

Elution Type

Isocratic

Mobile Phase

A: Acetonitrile

B: 100mM Ammonium Formate pH 3.2

C: H2O

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

80

10

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, HILIC 4 x 3.0 mm, 10/Pk Part No.: AJ0-8329 - Holder Part No.: KJ0-4282