Sterols from Peanut Butter by FID on ZB-MR2

GC Conditions

Column

Zebron ZB-MultiResidue-2, GC Cap. Column 30 m x 0.25 mm x 0.20 µm, Ea

Part No

7HG-G017-10

Phase Name

ZB-MultiResidue-2

Injection

Splitless @ 300°C

Carrier Gas

Constant Flow Helium, 2.0 mL/min

Detection

Flame Ionization (FID) @ (300°C)

Detection Info

Sample Notes

Sample Prep: Saponification, Solid Phase Extraction (SPE), and Derivatization.

Sample Preparation Details

Pretreatment Note

Initial Sample Weight / Unit: 0.70 grams

Condition 1

5.0 mL Methanol

Condition 2

5.0 mL Chloroform

Load

Load Sample

Elution 1

15 ml 5 % Methanol in Chloroform

Evaporation

Evaporate to dryness