Echinacea on Synergi Hydro-RP

LC Conditions

Column

Synergi 4 µm Hydro-RP 80 Å, LC Column 150 x 4.6 mm, Ea

Brand

Synergi

Part No

00F-4375-E0

Phase Name

Hydro-RP

Elution Type

Gradient

Mobile Phase

A: Water with 0.1% H3PO4

B: Acetonitrile with 0.1% H3PO4

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

20

75

25

Flow Rate

1.50 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info