PEGylated 1-7 fragment peptide Substance P on Jupiter® 300 C4 5µm, reaction at different time-points

LC Conditions

Column

Jupiter 5 µm C4 300 Å, LC Column 150 x 4.6 mm, Ea

Brand

Jupiter

Part No

00F-4167-E0

Phase Name

C4

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA 2% ACN in Water

B: 70/20% ACN/IPA 0.08% TFA in Water

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

85

15

2

25

30

70

Flow Rate

1.00 mL/min

Temperature

45°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (25°C)

Detection Info

Sample Notes

Separation of Amine PEGylated 1-7 fragment peptide Substance P (Arg-Pro-Lys-Pro-Gln-Gln-Phe) with 1kDa Methoxy PEG NHS-ester (PEG 2) on Jupiter 300 5µm C4.