LC/MS Separation poly dT 12-18 (Figure 5)

LC Conditions

Column

Clarity 3 µm Oligo-RP, LC Column 100 x 2 mm, Ea

Brand

Clarity LC

Part No

00D-4441-B0

Phase Name

Oligo-RP

Elution Type

Isocratic

Mobile Phase

A: 400mM HFIP/15mM TEA

B: 280mM HFIP/2.8mM TEA

C: 100mM HFIP/ 4mM TEA

D: 50mM HFIP/2mM TEA

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

Flow Rate

0.20 mL/min

Temperature

50°C

Detection

Mass Spectrometer (MS) @ (400°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Clarity Oligo-RP 4 x 2.0mm, 10/Pk Part No.: AJ0-8134 - Holder Part No.: KJ0-4282