LC/MS Separation poly dT 12-18 (Figure 3)

LC Conditions

Column

Clarity 3 µm Oligo-RP, LC Column 100 x 2 mm, Ea

Brand

Clarity LC

Part No

00D-4441-B0

Phase Name

Oligo-RP

Elution Type

Gradient

Mobile Phase

A: 100mM HFIP/4mMTEA/2% MeOH

B: 100mM HFIP/4mMTEA/2% MeOH

Gradient Profile

Step No.

Time(min)

%A

%B

1

0.01

95

5

2

1

95

5

3

5

50

50

4

5.5

40

60

5

6.5

20

80

6

7

95

5

7

10

95

5

Flow Rate

0.20 mL/min

Temperature

50°C

Detection

Mass Spectrometer (MS) @ (400°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Clarity Oligo-RP 4 x 2.0mm, 10/Pk Part No.: AJ0-8134 - Holder Part No.: KJ0-4282