Column Diameter Study with Luna 5u C18(2) 50 x 30mm

LC Conditions

Column

Luna 5 µm C18(2) 100 Å, LC Column 50 x 30 mm, AXIA Packed, Ea

Brand

Luna

Part No

00B-4252-U0-AX

Phase Name

C18(2)

Elution Type

Gradient

Mobile Phase

A: 0.5% TFA in Acetonitrile

B: 0.5% TFA in Water

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

5

95

2

5

95

5

Flow Rate

60.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

Comparision of column diameter on preparative separations using Axia-packed columns in various diameters: 4.6 (conventional hardware), 21.2, 30 and 50mm (cf: App IDs: 18062 - 18065). Load varied by diameter as follows: - 4.6mm: 64 µg in 21 µL DMSO (scaling factor = 1) - 21.2mm: 1.2 mg in 420 µL DMSO (scaling factor = 20) - 30mm: 2.4 mg in 840 µL DMSO (scaling factor = 40) - 50mm: 7.3 mg in 2100 µL DMSO (scaling factor = 118)