Tricyclic Antidepressants on Kinetex 2.6µm C18 50 x 2.1mm ID on Agilent 1200 (0.6 mL/min)

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4462-AN

Phase Name

C18

Elution Type

Isocratic

Mobile Phase

A: 20 mM Potassium Phosphate pH 7

B: 50:50 MeOH and ACN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

48

52

Flow Rate

0.60 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info