Tricyclic Antidepressants on Kinetex 2.6µm C18 50 x 4.6 mm ID on Agilent 1100 (system optimization)

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 4.6 mm, Ea

Brand

Kinetex

Part No

00B-4462-E0

Phase Name

C18

Elution Type

Isocratic

Mobile Phase

A: 20 mM Potassium Phosphate pH 7.0

B: 50:50 MeOH and ACN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

48

52

Flow Rate

2.90 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info