Extraction of Sulfonamides from Honey by LC/MS/MS Using strata X-C and Gemini 5u, C18, 150x3.0

Extraction of Sulfonamides from Honey by LC/MS/MS Using strata X-C and Gemini 5u, C18, 150x3.0
LC Conditions
Column

Gemini 5 µm C18 110 Å, LC Column 150 x 3 mm, Ea

Brand

Gemini

Part No

00F-4435-Y0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic acid/water

B: 0.1% formic acid/ACN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

8

30

70

Flow Rate

0.60 mL/min

Temperature

22°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info
Sample Notes

HPLC system: HP 1100 MS detector: API 3000, LC/MS/MS,Turbo spray: ESI, positive ion mode; MRM; heater gas flow 7000 cc/min; heater temp: 425° C

Sample Preparation Details
Description

Strata-X-C 33 µm Polymeric Strong Cation, 60 mg / 3 mL, Tubes, 50/Pk

Part No

8B-S029-UBJ

Pretreatment Note

Addition of IS followed by evaporation before reconstitution: Add IS (Sulfaquinoxaline) and evaporate the sample under slow stream of nitrogen @ 40 deg C and reconstitute with 100 uL of mobile phase

Condition 1

2 mL Methanol at 1 mL/min

Condition 2

2 mL Water at 1 mL/min

Load

Load 1.00 mL Sample diluted in 1mL of 2M HCl and 3mL 0.3M Citric acid

Wash 1

4 mL Water (2 aliquots of 2 mL) at 1 mL/min

Wash 2

4 mL MeOH/ACN (50/50) (2 aliquots of 2 mL) at 1 mL/min

Dry

2 mins at 10 of Hg pressure

Elution 1

2 mL 2% ammonium hydroxide/MeOH at 1 mL/min

Evaporation

Evaporate to dryness

Reconstitute

0 µL Mobile Phase

Order Items Used in This Application

Strata-X-C 33 µm Polymeric Strong Cation, 60 mg / 3 mL, Tubes, 50/Pk
8B-S029-UBJ

Strata-X-C 33 µm Polymeric Strong Cation, 60 mg / 3 mL, Tubes, 50/Pk

View Product

Gemini 5 µm C18 110 Å, LC Column 150 x 3 mm, Ea
00F-4435-Y0

Gemini 5 µm C18 110 Å, LC Column 150 x 3 mm, Ea

View Product

  • 1. Sulfanilamide
  • 2. Sulfathiazole
  • 3. Sulfamerazine
  • 4. Sulfamethoxazole
  • 5. Sulfaquinoxaline
-OEChem-06211003152D<br><br> 19 19  0     0  0  0  0  0  0999 V2000<br>    3.0000    1.5000    0.0000 S   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.0000    1.5000    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.0000    1.5000    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.0000    2.5000    0.0000 N   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.0000   -2.5000    0.0000 N   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.0000    0.5000    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.8660    0.0000    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.1340    0.0000    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.0000   -1.5000    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.8660   -1.0000    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.1340   -1.0000    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.4030    0.3100    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    1.5970    0.3100    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.4030   -1.3100    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    1.5970   -1.3100    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.5369    2.8100    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.4631    2.8100    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.4631   -2.8100    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.5369   -2.8100    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>  1  2  2  0  0  0  0<br>  1  3  2  0  0  0  0<br>  1  4  1  0  0  0  0<br>  1  6  1  0  0  0  0<br>  4 16  1  0  0  0  0<br>  4 17  1  0  0  0  0<br>  5  9  1  0  0  0  0<br>  5 18  1  0  0  0  0<br>  5 19  1  0  0  0  0<br>  6  7  2  0  0  0  0<br>  6  8  1  0  0  0  0<br>  7 10  1  0  0  0  0<br>  7 12  1  0  0  0  0<br>  8 11  2  0  0  0  0<br>  8 13  1  0  0  0  0<br>  9 10  2  0  0  0  0<br>  9 11  1  0  0  0  0<br> 10 14  1  0  0  0  0<br> 11 15  1  0  0  0  0<br>M  END
Compound Name

Sulfanilamide

CID

5333

Molecular Formula

C6H8N2O2S

Molecular Weight

172

No. Hydrogen Bond Acceptors

4

No. Hydrogen Bond Donors

2

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