Steroids on Kinetex 2.6µm C18 50 x 2.1mm ID (in acetonitrile)

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Water

B: CH3CN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

80

20

2

10

50

50

3

10.1

80

20

4

12

80

20

Flow Rate

0.80 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (25°C)

Detection Info