Extraction of Carbamate Pesticides using Strata-X on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 5mM Ammonium formate in H2O

B: mM Ammonium formate in ACN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

2

0.25

97

3

3

2.5

5

95

4

4

5

95

5

4.01

97

3

6

5.5

97

3

Flow Rate

500.00 mL/min

Temperature

0°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Preparation Details

Description

Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 3 mL, Tubes, 50/Pk

Part No

8B-S100-TBJ

Condition 1

1 mL Methanol at 2 mL/min

Condition 2

1 mL 25 mM ammonium acetate pH 5.5 at 2 mL/min

Load

Load 0.40 mL Sample diluted in 1

Wash 1

1 mL 25 mM ammonium acetate pH 5.5 at 2 mL/min

Wash 2

1 mL 15% methanol at 2 mL/min

Dry

2 mins at 10 of Hg

Elution 1

0.5 mL 5% formic acid/methanol at 1 mL/min

Elution 2

0.5 mL 5% formic acid/methanol at 1 mL/min

Evaporation

Evaporate to dryness

Reconstitute

200 µL 97/3 initial LC mobile phase